Earlier, reports surfaced that Prime Minister Shahid Khaqan Abbasi advised him to step down allegedly on counsel by Nawaz Sharif.
Zehri's resignation was promptly accepted by Balochistan Governor Muhammad Khan Achakzai.
The unending episode of submitting resignations by the provincial cabinet members, advisers and secretaries began in the first of January after a no-trust motion against Zehri was moved in the provincial assembly. He added that he was not in the habit of hanging onto power at any cost.
The chief minister of Pakistan's southwestern province of Balochistan has resigned amid opposition lawmakers' efforts to oust him through a vote of no confidence.
In order to avoid divisions within the party ranks and imminent "horse-trading" in the provincial assembly, he should leave the post before the no-confidence motion, said Mr. Abbasi.
"That is in the party's interest", he added. Soon after his sacking, the former home minister, however, claimed that he had submitted resignation and the reports of his dismissal were untrue.
PM Abbasi advised Zehri to resign after the dissenting leaders rejected his invitations to discuss outstanding issues, sources within the PML-N told Pakistan Today.
Speaking to Geo News prior to the resignation, Balochistan Opposition Leader Maulana Wasay said it would be better if the chief minister accedes to the prime minister's advice.
At least 33 votes are required in the 65-member house to topple the government and both sides are claiming of having enough support to serve their respective causes.
Some members of the PML-N, too, had refused to meet with Abbasi.
Earlier, Sarfraz Bugti, the former home minister of Pakistan's biggest land-wise province took to the micro-blogging site and expressed gratitude over the decision. We will surely approach Sanaullah Zehri for his vote for the election of the new chief minister. The motion was expected to be taken up in the assembly today at 4 pm The Pakhtunkhawa Milli Awami Party, the ruling party's ally in Balochistan, has 14 seats in the assembly, while, the National Party has 11 seats.